Glucosylation of the 21-hydroxyl group of glucocorticoid changes its solubility into hydrophilicity from hydrophobicity and, as with glucocorticoid glucuronides as a moving object in vivo, it is conceivable virginia mill works tobacco road acacia that it exhibits the same behavior.Therefore, glucosylation to the 21-hydroxyl group while maintaining the 11β-hydroxyl group is particularly important, and glucosylation of corticosterone was confirmed by high-resolution mass spectrometry and 1D (1H and 13C) and 2D (COSY, ROESY, HSQC-DEPT and HMBC) NMR.Moreover, the difference in bioactivity between corticosterone and corticosterone 21-glucoside was investigated in vitro.
Corticosterone 21-glucoside showed greater neuroprotective effects against H2O2-induced cell death and turbo air m3f24-1 reactive oxygen species (ROS) compared with corticosterone.These results for the first time demonstrate that bioconversion of corticosterone through the region-selective glucosylation of a novel compound can present structural potential for developing new neuroprotective agents.